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DTSTART;TZID=Europe/Paris:20260323T110000
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DTSTAMP:20260404T114055
CREATED:20260320T094540Z
LAST-MODIFIED:20260320T094546Z
UID:10000101-1774263600-1774267200@sfp-alpes.fr
SUMMARY:Chloé ROFFAY (IMP Vienna\, Austria)
DESCRIPTION:Uncovering the forces driving the fate and shape of the extraembryonic amnion during human gastrulation\nRésumé : \nExtraembryonic tissues provide key molecular signals and mechanical support to the growing embryo. For instance\, the extraembryonic amnion\, which forms a fluid-filled sac surrounding the embryo\, was recently shown to trigger germ layer specification during gastrulation\, by secreting BMP ligands. Despite the key roles of extraembryonic tissues in embryo development\, little is still known regarding their molecular and biophysical programs\, particularly in human. Using a 2D stem cell-based model of human gastrulation\, termed gastruloid discs\, we found that amnion cells undergo a sharp columnar-to-squamous transition concomitantly with fate specification. Via biophysical modelling\, direct force measurements\, pharmacological and genetic perturbations\, we showed that this morphogenetic transition is amnion-intrinsic and it is driven by active wetting\, i.e. a transition from tensile to adhesion-dominated cellular states. Molecularly\, active wetting is implemented via a rewiring of cytoskeleton composition\, from actomyosin to keratin-based cytoskeletal networks\, akin to a bistable toggle-switch in gene regulatory networks. Strikingly\, blocking shape changes at the colony edge results both in defective cellular states in the amnion and impaired gastruloid disc morphogenesis within the embryonic compartment. Together\, our findings establish that a cytoskeletal toggle switch couples fate specification to tissue architecture in the human amnion and suggest an unexpectedly active mechanical role for extraembryonic tissues in shaping the embryo proper. \nContact : thomas.boudou@univ-grenoble-alpes.fr
URL:https://sfp-alpes.fr/event/chloe-roffay-imp-vienna-austria/
LOCATION:LiPhy – Salle de conférence\, LiPhy 140 avenue de la Physique\, St Martin d'Hères\, 38402\, France
CATEGORIES:Séminaire
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