Versatility of Melanoma antigens in regulating human ubiquitin signalling

Résumé :

Humans carry around 40 different genes coding for melanoma antigen (MAGE) proteins. Most MAGE proteins are selectively expressed in the testis and cancer, categorizing them as cancer-testis antigens (CTAs). These CTA MAGEs are potently oncogenic and known to be poor prognostic markers. Additionally, around 11 MAGE proteins are expressed in various tissues, with a particular enrichment in the brain. MAGEs interact with multiple ubiquitin ligases to influence their activity or redirect them to specific substrates in a context-dependent manner. We use integrative structural biology techniques combined with proteomics and light microscopy to answer fundamental questions in MAGE biology. In this talk, I will present two examples of MAGE-driven signaling—one involving an oncogenic MAGE and another involving a neuronal-specific MAGE protein. I will also present our efforts to develop novel, proteomics-based methods to study ubiquitin signaling at scale.

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